小腸耶爾森菌核酸PCR檢測試劑盒

廣州健侖生物科技有限公司

廣州健侖長期供應各種流感檢測試劑，包括進口和國產的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、凱必利、廣州創侖等。

產品名稱：小腸耶爾森菌核酸PCR檢測試劑盒；

 產品品牌：創侖；

 產品用途：本PCR-熒光探針法體外定性檢測；

 產品規格：24T/盒； 

 保存條件：避光 -20度 保存。

    我司還提供各種流感病毒、副流感病毒、呼吸道合胞病毒、冠狀病毒、輪狀病毒、桿菌、鏈球菌、熱帶病毒等等核酸檢測試劑盒（PCR-熒光探針法），隨時歡迎您的來電

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

以下是我司提供的部分PCR產品

1. background of research

Grass carp (Ctenopharyngodon idella), also known as grass carp, grass carp, black, dronish, inhabits plains rivers and lakes. Aeromonas hydrophila (Aeromonas hydrophila) is widely distributed in all kinds of water bodies in nature, and is the primary pathogenic bacteria of various aquatic animals. For a long time, grass carp on Aeromonas hydrophila is susceptible, infected individuals have a higher mortality rate, resulting in large economic losses to the fishery resources. Aeromonas hydrophila invaded the fish body and then proliferated in the intestine first, then entered the liver, kidney and other tissues through the portal artery circulation, causing liver, kidney and other organs and blood diseases, followed by systemic symptoms. Then, from grass carp Aeromonas infection, the immune system of organism grass carp is how to make the response, gene mRNA level is few.

2. purpose of research

RNA-Seq research shows that a variety of fish species such as giant salamander, Qinghai Lake naked fish, turbot was Aeromonas hydrophila infection, the structure and function of immune related gene expression changes. In order to study from grass carp Aeromonas infection, immune response mechanism in the development of grass carp, Ocean University of China, Key Laboratory of aquatic resources and utilization of the party on the flying cloud infection before and after different time grass carp spleen by transcriptome sequencing.

3. research methods

The grass carp research sample collected from Jiangsu Wujiang state farms, breeding in circulating water with high k * * sterilization, three times a day feeding. The experimental group grass carp intraperitoneal injection of 100ul 2.7 * 107 CFU/mL of Aeromonas hydrophila, control group were injected with the same volume of grass carp PBS. The experimental group grass carp injection of Aeromonas hydrophila after 4, 8, 12, 24, 48 and 72 h respectively taken fish spleen tissue, and immediay frozen in liquid nitrogen and stored in the -80 C refrigerator. After each sample, the total RNA was extracted and sequenced.

4. analysis content and result explanation

4.1 sequence splicing and UniGene functional annotation

Through the analysis of RNA-seq non reference transcriptional group data, the sequence splicing was 52668 unigenes, the average length was 1072bp