流感診斷血清 a型2ml

廣州健侖生物科技有限公司

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流感診斷血清 a型2ml

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

實驗步驟
1. 破壞腦脊髓：取蟾蜍一只，用自來水沖洗干凈。左手握住蟾蜍，用食指壓住頭部前端使頭前俯，右手持刺蛙針從枕骨大孔向前刺入顱腔，左右攪動搗毀腦組織，然后將刺蛙針退到枕骨大孔，不拔出而是將其尖轉向后插入脊柱管中搗毀脊髓，插入椎管時，蟾蜍后肢立即失去緊張性，多數情況出現尿失禁。若腦脊髓破壞*，可見蟾蜍四肢松弛，呼吸消失。
2. 剪除上肢和內臟：在骶髂關節上0.5～1.0cm處用粗剪刀剪斷脊柱。用鑷子夾住后端脊柱，以剪刀沿脊柱兩側剪除所有內臟及頭胸口，留下后肢、骶骨、后端脊柱及緊貼于脊柱兩側的坐骨神經。
3. 剝皮：左手用鑷子或直接用手捏住脊柱斷端（注意不要壓迫神經），右手捏住斷端邊緣皮膚，向下剝去全部后肢皮膚，將標本置于盛有任氏液的培養皿中。將手和用過的器械洗凈后再進行以下步驟。
4. 分離兩腿：用玻璃分針沿脊柱兩側游離出兩條坐骨神經，并于近脊柱處各扎一細線，然后在扎線與脊柱之間剪斷神經。提著神經上的細線，將兩條坐骨神經分別置于兩條大腿上，左手持脊柱，將骶骨翹起，將下位脊柱全部剪除。捏著兩側髂骨向反方向分離，使恥骨聯合脫臼后，沿恥骨聯合正中將兩下肢剪開，將一條腿浸于任氏液中備用，另一條置于浸有任氏液的玻璃板上。
5. 游離坐骨神經和剪斷股骨：認清坐骨神經溝和腓腸肌的部位，用剪刀剪斷梨狀肌及其周圍的結締組織，左手提著神經上的細線，右手持剪刀或玻璃分針沿坐骨神經溝細心剝離，直至將坐骨神經剝離到腘窩。將游離干凈的坐骨神經放在下腿上，沿膝關節的周圍將大腿的所有肌腱剪斷，并用剪刀刮凈股骨下段附著的肌肉，在股骨上1/3處剪去上段股骨及所附的肌肉，這樣制成的稱為坐骨神經下腿標本。
6. 游離腓腸肌：在坐骨神經下腿標本的基礎上，用剪刀將跟腱的下端剪斷，在跟腱與肌肉交界處扎一條細線，左手提線，右手用剪刀游離腓腸肌，直到膝關節。zui后用粗剪刀在膝關節下將小腿剪去，留下的即為坐骨神經腓腸肌標本。做好的標本用鋅銅叉的兩極輕輕接觸坐骨神經，如腓腸肌立即收縮，表示標本的興奮性良好，然后將標本放入任氏液中，待其興奮性穩定后再進行實驗。

Experimental steps
1. Destruction of the brain spinal cord: take a toad, rinse with tap water. Hold the toad with his left hand, press the front of the head with the index finger to head down, the right hand holding the frog needle piercing the foramen magnum foramen into the cranial cavity, left and right stir to destroy the brain tissue, and then frog needle back to the foramen magnum, not Pull out but pointed into the spine after turning into the spinal canal to destroy the spinal cord, insert the spinal canal, toad hindlimbs immediay lose tension, urinary incontinence in most cases. If the destruction of the brain spinal cord compley, we can see the toads relaxed limbs, breathing disappeared.
2. Cut off the upper extremities and internal organs: 0.5 ~ 1.0cm in the sacroiliac joint with a coarse scissors to cut the spine. Clamp the posterior spine with tweezers, cut off all the viscera and head chest with scissors on both sides of the spine, leaving hind limbs, sacrum, posterior spine and sciatic nerve in close contact with both sides of the spine.
3. Skinning: left hand tweezers or pinch the spine directly with your hands (be careful not to pressure the nerve), the right hand pinch the edge of the broken edge of the skin, peel downward all the skin of the hind limbs, Dish. Wash hands and used instruments before proceeding with the following steps.
4. Separation of legs: with glass minutes along both sides of the spine free two sciatic nerve, and in the proximal spinal column at each tie a thin line, and then cut the nerves between the nails and spine. Carrying the nerve on the thin line, the two sciatic nerve were placed on two thighs, left hand holding the spine, the sacrum tilt, the lower spine all cut off. Pinch the iliac on both sides of the separation to the opposite direction, the dislocation of the pubic symphysis, the middle of the pubic symphysis will lower both limbs, one leg immersed in Ren liquid spare, the other placed in Ren's liquid immersed glass on.
5. Free sciatic nerve and cut the femur: recognize the parts of the sciatic nerve sulcus and gastrocnemius muscle, with the scissors to cut the piriformis and its surrounding connective tissue, the left hand carrying the nerve on the thin line, right hand holding scissors or glass minute hand along the sciatic nerve groove carefully peel , Until the sciatic nerve stripped to popliteal fossa. The free and clean sciatic nerve on the lower leg, along the knee around the thigh will be cut all the tendons, and use scissors to clean the muscles attached to the lower femur, the upper femur 1/3 cut off the upper femur and attached muscles, so Made of sciatic nerve lower leg specimens.
6. Free gastrocnemius: On the basis of sciatic nerve lower leg specimens, cut the lower end of the Achilles tendon with scissors, tie a thin line at the junction of Achilles tendon and muscle, raise the left hand and the right hand use scissors to free the gastrocnemius muscle until the knee joint. Finally, use the scissors to cut off the calf under the knee joint, leaving the sciatic nerve gastrocnemius specimen. Well done specimens with zinc copper fork gently touch the sciatic nerve poles, such as immediate contraction of the gastrocnemius, said specimens of excitability is good, and then the specimens into Ren's solution, to be excitatory stability after the experiment.